We provide contract glycoanalytics, contract research and fee-for-service products helping to boost your product development efforts, from initial discovery phase and R&D to clinical trials and eventual product approval.
Our high-performance glycoanalytical services are individualized according to specific project requirements to best serve the needs of our clients coming from a variety of fields spanning science, medicine and industry.
In addition to our services, we also offer a broad range of glycoanalytical products and tools including sample preparation kits, glycan standards, software tools and more.
Visit our Products information page to learn more about glyXera’s product catalog.
Glycan Analysis
Services
High-Performance Glycan Analysis Solutions
We specialize in glycomic and glycoproteomic analysis of samples from a wide variety of biological and biotechnological sources.
Versatile Analytical Workflows
Our expertise covers a wide range of glycoanalytical approaches which can be flexibly adapted to accommodate individual project requirements.
We specialize in high-throughput and in-depth glycoprofiling using our patented glyXbox™ technology platforms, delivering unprecedented performance and cost-effective scalability.
Our glycoprofiling offering is supplemented by additional glycoanalytical techniques such as comprehensive glycopeptide mapping, general characterization of intact glycoproteins, and more.
Glycoprofiling
Glycoprofiling is provided for purified glycoproteins, protein mixtures (separated by 1D or 2D gel electrophoresis), or from complex samples.
We provide high-performance glycoprofiling with three different levels of sophistication
Basic
Glycofingerprinting
Our exclusive glyXbox™ glycoanalysis platform generates highly reproducible results, thereby uncovering each sample’s characteristic glycomic composition pattern – its Fingerprint.
Comparative analysis of Fingerprints aligned via our patented orthogonal double alignment method provides reliable overviews of the glycomic macro-heterogeneity of samples.
Standard
Glycoprofiling
Annotating a Fingerprint’s picked and integrated peaks with glycan structures yields a sample’s distinctive Glycoprofile.
Structural assignments are obtained via matching aligned peak positions to entries in a comprehensive glycan database. The assignment results are further refined by taking into account a priori knowledge about the sample material.
Extended
Glycoprofiling
Further insights into the structural composition of particularly complex Glycoprofiles are gained via a combination of exoglycosidase treatments and repeated glycoprofiling.
Comparative evaluation of Glycoprofiles obtained from exoglycosidase experiments reveals additional in-depth structural information such as sialic acid linkages, isoforms, and more.
Glycopeptide Mapping
for determination of glycosylation sites and glycan heterogeneity, gained using high-resolution LC‑ESI‑MS.
Site Occupancy Analysis
of N- or O‑linked glycosylation sites of monoclonal antibodies or other recombinant glycoproteins.
Intact Glycoprotein Analysis
via molecular mass determination of intact protein glycoforms, gained using high-resolution CE‑MS or LC‑MS.
Further analytical techniques on offer
- Monosaccharide analysis consisting of separation and quantification of enzymatically or chemically released monosaccharides, e.g. terminal sialic acids or alpha galactoses.
- Protein identification obtained via peptide fragment mass fingerprinting using tandem mass spectrometry.
- General glycoprotein characterization achieved using protein gel electrophoresis pattern analysis of one- or two-dimensional gels.
Consultancy
Services
Professional Laboratory Problem Solving and Guidance
With decades of combined glycoanalytical experience, our team of experts stands ready to assist you in planning, implementing and optimizing laboratory procedures and to guide you through all phases of product development.
We help you to select the best cell line regarding the desired glycosylation pattern of your target as well as to establish the best glycoanalytical approaches and methods.
In addition, we assist you in planning and designing your studies, for instance by identifying possible sources of contamination in your samples and advising you how to adapt your experimental setup accordingly.
We offer comprehensive glycoanalytical training programs for your team, covering all topics ranging from sample preparation to final data analysis.
Our training services are individually tailored to meet your specific needs and requirements and can be provided either on-site or remotely.
We provide our technical expertise to qualify and maintain your glycoanalytical systems and to assist in troubleshooting technical issues with your experimental setups.
Furthermore, we will advise you about which analytical systems, laboratory devices and workflows are best suited for your specific glycoanalytical purposes.