Our glyXprep sample preparation kit contains all required materials to facilitate effortless, straightforward and reproducible N‑glycan profiling (analysis of N‑glycans released from glycoproteins).
The sample preparation procedure consists of three separate modules:
N‑Glycan Release: In‑solution deglycosylation of purified glycoproteins or whole glycoprotein mixtures by PNGase F
N‑glycan Labeling: Selective tagging of released N-glycans with fluorescent dye APTS
HILIC‑SPE Clean‑Up: Post-labeling clean‑up of APTS‑labeled N-glycans by hydrophilic interaction liquid chromatography based solid phase extraction
glyXprep is available in several size options – for 16, 48 or 96 samples. Discounts can be offered for bulk purchases.
glyXprep is optimized for use with our high‑performance glyXboxCE glycoanalysis system, but is also compatible with various other separation-based N‑glycan analysis techniques, including:
- Capillary Zone Electrophoresis (CZE)
- Hydrophilic Interaction High Performance Liquid Chromatography (HILIC‑HPLC)
- Hydrophilic Interaction Ultra Performance Liquid Chromatography (HILIC‑UPLC)
For questions regarding instrument or chromatography column compatibility, please contact our support.
Contents |
16 Samples | 48 Samples | 96 Samples | |
| Release | Denaturation Solution | 50 µL | 100 µL | 200 µL |
|---|---|---|---|---|
| Neutralization Solution | 100 µL | 200 µL | 400 µL | |
| PNGase F Solution | 20 µL | 55 µL | 110 µL | |
| Positive Control | 20 µL | 40 µL | 80 µL | |
| Labeling | Ultrapure Water | 200 µL | 250 µL | 500 µL |
| APTS Solution | 40 µL | 110 µL | 220 µL | |
| ReduX Solution | 40 µL | 110 µL | 220 µL | |
| Stopping Solution | 1.8 mL | 5.4 mL | 10.8 mL | |
| Clean-Up | Washing Solution I | 30 mL | 100 mL | 200 mL |
| Washing Solution II | 20 mL | 70 mL | 140 mL | |
| glyXbeads Slurry | 5 mL | 15 mL | 30 mL | |
| Elution Solution | 30 mL | 100 mL | 200 mL | |
| HILIC‑SPE Filter Plate | 1 pc | 1 pc | 2 pcs | |
| Waste Plate | 2 pcs | 2 pcs | 4 pcs | |
| Deep Well Plate | 1 pc | 1 pc | 2 pcs | |
| Plate Sealing Tape | 1 pc | 2 pcs | 4 pcs |
The use of the kit requires a vacuum manifold system, which must be ordered separately from the manufacturer.
For optimal results, we recommend using 5‑100 µg of pure glycoproteins or 1‑6 µL of glycoprotein mixtures.
