We are pleased to announce that glyXera, together with our research partners at the University of Heidelberg and the University of Copenhagen, published a new research article in the journal Molecular Genetics and Metabolism.
The publication, titled “Truncated N-glycans destabilize POMT1 and POMT2 but do not limit cellular O-mannosylation in HEK293 cells”, shows how a defect in the N-glycosylation pathway impacts other glycosylation pathways. This work established a disease model for congenital disorder of glycosylation (CDG) type Id, that is characterized by a deficiency in the enzyme ALG3. ALG3 is a mannosyltransferase involved in the synthesis for lipid-linked N-glycan precursors. Here, the impact of this deficiency on the POMT1 and POMT2 enzymes, responsible for O-mannosylation, were explored.
Using glyXera’s multiplexed capillary gel electrophoresis with laser-induced fluorescence (xCGE-LIF) technology, paucimannose type N-glycans, typically found as lipid-linked precursors in the endoplasmic reticulum, were identified on proteins and easily distinguished from the usual isomers appearing in the Golgi pathway. Furthermore, an xCGE-LIF-based workflow was used for semi-quantitative comparison of N-glycan levels in the disease model and wild type cells.
The article is published open access and can be read in full on the publication web page: https://www.sciencedirect.com/science/article/pii/S1096719226003987
If you would like to learn more about our high-performance glycan analysis products, including glyXboxCE, or are interested in a research cooperation, please feel free to get in touch.