glyXera is pleased to announce the publication of its contributed chapter “Capillary (Gel) Electrophoresis-Based Methods for Immunoglobulin (G) Glycosylation Analysis” as part of the Springer Book “Antibody Glycosylation”, edited by Marija Pezer.
Immunoglobulins, also called antibodies, are blood glycoproteins which serve as one of the first defense mechanisms against pathogens in vertebrates. These antibodies exist in several forms with different functions as a part of the adaptive immune system. In humans, immunoglobulins are divided into the five main classes IgA, IgD, IgE, IgM, and the very well-known IgG. All have one characteristic in common: their glycosylation is of particular importance for their appropriate function and stability. However, except for the well-characterized IgG, the glycan–function relationship is still not fully understood.
Our chapter provides a comprehensive overview of the latest developments in capillary (gel) electrophoresis-based glycan analysis. It addresses topics like miniaturization (microchip CE), CE coupling to mass spectrometry, as well as performance, limitations, and possibilities of CE/CGE-based glycan analysis. Furthermore, the chapter includes an application section, comparing species-specific IgGs, human immunoglobulins, and therapeutic monoclonal antibodies from different production cell lines. Altogether, the chapter illustrates the full potential of CE/CGE-based glycan analysis methods and serves as an in-depth guide to antibody glycosylation with its many descriptive examples.
The book chapter is published open access and can be read in full on the publication web page: https://link.springer.com/chapter/10.1007/978-3-030-76912-3_4
If you would like to learn more about glyXera’s capillary (gel) electrophoresis-based glycan analysis technologies and their applications in glycoprotein characterization, or if you are interested in our tailored glycoanalytical services, please do not hesitate to get in touch with us.