MIRAGE (Minimum Information Required for A Glycomics Experiment) is an international scientific initiative, coordinated by the Beilstein-Institut, to standardize the reporting of glycoanalytical methods and to assess their reproducibility. published several reporting guidelines that describe what information should be provided for sample preparation methods, mass spectrometry methods, liquid chromatography analysis, exoglycosidase digestions, glycan microarray methods, and nuclear magnetic resonance methods.
Among the latest MIRAGE publications are the reporting guidelines for glycomics analysis by capillary electrophoresis (CE) for standardized and high-quality reporting of experimental conditions in scientific literature. The guidelines cover all aspects of a glycomics CE experiment including sample preparation, CE operation mode, instrument configuration, capillary separation conditions, detection, data analysis, and experimental descriptors, to assist scientists to improve their reporting and the reproducibility of their glycomic methods using CE.
The guidelines are published in Glycobiology and also available on the MIRAGE project website: https://www.beilstein-institut.de/en/projects/mirage/guidelines/#ce_analysis
Among glyXera’s contributions to the guidelines is a data reporting example using xCGE-LIF based on a previously published longitudinal study on the long-term stability of the human plasma N-glycome (Hennig, Čajić, et al., 2016).
glyXera has a wide variety of glycoanalytical products and services on offer that are compatible with the MIRAGE guidelines. If you would like to learn more about our product range or are interested in enlisting glyXera’s help in adapting your own glycoanalytical methods to conform to MIRAGE standards, feel free to get in touch.